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PulseNet is a national laboratory network that connects cases of foodborne illnesses cases together using DNA fingerprinting of the bacteria making people sick. Various shots show a microbiologist going through the process of one type of DNA fingerprinting technique, pulsed-field gel electrophoresis (PFGE). The last few clips show database managers analyzing these DNA fingerprints in the national databases in order to detect and define outbreaks.

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In this activity, students set up an alcoholic fermentation, prepare and view a slide of bacteria responsible for monolactate fermentation and answer questions about a video clip on gut bacteria fermentation.

It has been estimated that there are more bacteria in the large intestine than there are cells in the body. Maintaining a healthy bacterial population in the large intestine plays a key role our sense of wellbeing. Use our article Healthy gut bacteria to explore this further.

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In both infective and noninfective endocarditis, accumulations of blood clots (and bacteria in infectious endocarditis) can break free from the heart wall (becoming emboli), travel through the bloodstream, and block an artery. This blockage may cause a stroke Overview of Stroke A stroke occurs when an artery to the brain becomes blocked or ruptures, resulting in death of an area of brain tissue due to loss of its blood supply (cerebral infarction) and symptoms that... read more or damage to the spleen, kidneys, or other organs.

Bacteria (or, less often, fungi) that are introduced into the bloodstream can sometimes lodge on heart valves and infect the endocardium. Abnormal, damaged, or replacement (prosthetic) valves are more susceptible to infection than normal valves. The bacteria that cause subacute bacterial endocarditis nearly always infect abnormal, damaged, or replacement valves. However, normal valves can be infected by some aggressive bacteria, especially if many bacteria are present.

Although bacteria are not normally found in the blood, an injury to the skin, lining of the mouth, or gums (even an injury from a normal activity such as chewing or brushing the teeth) can allow a small number of bacteria to enter the bloodstream. Gingivitis Gingivitis Gingivitis is a mild form of periodontal disease characterized by inflammation of the gums (gingivae). Gingivitis results most often from inadequate brushing and flossing but may result from... read more (inflammation of the gums) with infection, minor skin infections, and infections elsewhere in the body may introduce bacteria into the bloodstream.

Sepsis Sepsis and Septic Shock Sepsis is a serious bodywide response to bacteremia or another infection plus malfunction or failure of an essential system in the body. Septic shock is life-threatening low blood pressure ... read more , a severe blood infection, introduces a large number of bacteria into the bloodstream. When the number of bacteria in the bloodstream is large enough, endocarditis can develop, even in people who have normal heart valves.

Acute bacterial endocarditis usually begins suddenly with a high fever (102 to 104F [38.9 to 40C]), fast heart rate (> 100 beats per minute), fatigue, and rapid and extensive heart valve damage causing symptoms of heart failure Symptoms Heart failure is a disorder in which the heart is unable to keep up with the demands of the body, leading to reduced blood flow, back-up (congestion) of blood in the veins and lungs, and/or... read more .

Subacute bacterial endocarditis may cause such symptoms as fatigue, mild fever (99 to 101 F [37.2 to 38.3C]), a moderately fast heart rate, weight loss, sweating, and a low red blood cell count (anemia Overview of Anemia Anemia is a condition in which the number of red blood cells is low. Red blood cells contain hemoglobin, a protein that enables them to carry oxygen from the lungs and deliver it to all parts... read more ). These symptoms can be subtle and may occur for months before endocarditis results in blockage of an artery or damages heart valves and thus makes the diagnosis clear to doctors.

In both acute and subacute bacterial endocarditis, arteries may become blocked if accumulations of bacteria and blood clots on the valves (called vegetations) break loose (becoming emboli), travel through the bloodstream to other parts of the body, and lodge in an artery, blocking it. Sometimes blockage can have serious consequences. Blockage of an artery to the brain can cause a stroke Overview of Stroke A stroke occurs when an artery to the brain becomes blocked or ruptures, resulting in death of an area of brain tissue due to loss of its blood supply (cerebral infarction) and symptoms that... read more , and blockage of an artery to the heart can cause a heart attack Acute Coronary Syndromes (Heart Attack; Myocardial Infarction; Unstable Angina) Acute coronary syndromes result from a sudden blockage in a coronary artery. This blockage causes unstable angina or a heart attack (myocardial infarction), depending on the location and amount... read more . Emboli can also cause an infection in the area in which they lodge and/or block small blood vessels and damage organs. Organs that are often affected include the lungs, kidneys, spleen, and brain. Emboli also often travel to the skin and back of the eye (retina). Collections of pus (abscesses) may develop at the base of infected heart valves or wherever infected emboli settle.

To help make the diagnosis, doctors usually do echocardiography and obtain blood samples to test for the presence of bacteria. Usually, three or more blood samples are taken at different times on the same day. These blood tests (blood cultures Culture of Microorganisms Infectious diseases are caused by microorganisms, such as bacteria, viruses, fungi, and parasites. Doctors suspect an infection based on the person's symptoms, physical examination results,... read more ) may identify the specific disease-causing bacteria and the best antibiotics to use against them. In people with heart abnormalities, doctors test their blood for bacteria before giving them antibiotics.

Echocardiography Echocardiography and Other Ultrasound Procedures Ultrasonography uses high-frequency (ultrasound) waves bounced off internal structures to produce a moving image. It uses no x-rays. Ultrasonography of the heart (echocardiography) is one of... read more , which uses ultrasound waves, can produce images showing heart valve vegetations and damage to the heart. Typically, transthoracic echocardiography (a procedure in which the ultrasound probe is placed on the chest) is done. If this procedure does not provide enough information, the person may undergo transesophageal echocardiography (a procedure in which the ultrasound probe is passed down the throat into the esophagus just behind the heart). Transesophageal echocardiography is more accurate and detects smaller bacterial deposits, but it is invasive and more costly.

Sometimes bacteria cannot be cultured from blood samples. Special techniques may be needed to grow the particular bacteria, or the person may have taken antibiotics that did not cure the infection but did reduce the number of bacteria enough to be undetectable. Another possible explanation is that the person does not have endocarditis but has another condition, such as a heart tumor Overview of Heart Tumors A tumor is any type of abnormal growth, whether cancerous (malignant) or noncancerous (benign). Tumors in the heart may be Primary (noncancerous or cancerous) Metastatic (always cancerous) Primary... read more or noninfective endocarditis Noninfective Endocarditis Noninfective endocarditis is formation of blood clots on heart valves and the lining of the heart. Symptoms occur when a blood clot breaks loose and blocks arteries elsewhere in the body. Diagnosis... read more , that causes symptoms very similar to those of endocarditis.

Antibiotics alone do not always cure an infection, particularly if the valve is one that has been replaced. One reason is that the bacteria that cause endocarditis in a person with a replacement valve are often resistant to antibiotics. Because antibiotics are given before heart valve replacement surgery to prevent infection, any bacteria that survive this treatment to cause infection are probably resistant. Another reason is that it is generally harder to cure infection on artificial, implanted material than in human tissue.

Doctors may use a series of echocardiography examinations to ensure that the infected area is decreasing. They may also do echocardiography at the end of treatment to have a record of the appearance of heart valves because infective endocarditis may recur. Because of the risk of recurrence, ongoing dental care and good skin hygiene (to prevent any bacteria from entering the body through sores or wounds) is needed.

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Did you know that 20% of the oxygen we breathe comes from the ocean? Tiny bacteria called prochlorococcus are hard at work turning carbon dioxide into the oxygen we breathe through photosynthesis. Join oceanographer and National Geographic Explorer-in-Residence Sylvia Earle to learn about this small but mighty organism.